I. Buffers
1) Protein gel
a. 30% Acrylamide stock
b. 10x RBG
c. 4x SBG
d. SDS-PAGE running buffer
e. 18% acrylamide gel
f. 10% acrylamide gel
g. 4.8% stacking gel
h. 4x SSB
i. Tris-tricine
2) DNA work
a. 50x TAE
b. 5x TBE
c. 10x TE
d. 10x loading dye
3) Western blot
a. 10x TBS
b. 1x TBST
c. 1x TBST NFM
4) Chloroplast buffer
a. 2x Grinding buffer
b. 2x Import buffer
c. 5x Import buffer
d. 40% Percoll
e. 80% Percoll
5) General Buffer
a. 1M Tris-HCl
b. 0.5M EDTA pH 8.0
II. Gel Protocols
1) DNA gel
a. Agarose
b. Acrylamide
2) Protein gel
a. small gel setup
b. large gel setup
c. SDS-PAGE
d. Tris-tricine
e. gradient gel (small, large gel)
3) Western analysis (blot, antibody, detection)
4) Strom Phosphoimager (Hot gel)
III. Plant Protocols
1) Grow (germination, growth condition)
a. pea
b. arabidopsis
c. tobacco
2) Transformation
a. particle gun
b. agrobacterium infiltration
c. FAST method
d. Floral dip
3) DNA extraction
4) Total protein extraction
IV. Microbiology Protocols
1) Media
a. LB
b. Terrific Broth
c. SV
d. etc....
2) Glycerol Stock/Cryogenic
3) Transformation (cell prep & transform)
a. E. coli
b. Agrobacterium
c. Ify conjugation
d. Daniel electroporation
4) Fermenter culture/ cleaning
5) etc....
V. Chloroplast Protocols
1) Chloroplast prep, full
2) Chloroplast prep, short
3) Envelop prep
4) Stromal extract prep
5) CSS1 prep
6) Import Assay
7) Binding Assay
9) In vivo import quantification
10) Ashita's protocols (antibody, cross linking, FACS etc)
11) Evan's protocols (GTPase assay)
VI. Protein Protocols
1) Impact protein expression purification
a. induction screen
b. glycerol stock
c. expression & purification using chitin column
d. expression & purification using HPLC (Richard)
2) Cobalt sepharose
3) TALON
4) Cytochrome purification
5) AUC
6) Richard protocols (pull down, cross linking)
7) MIANS labeling (prep, clean up)
8) Lyophilization (machine setup)\
9) MALDI
10) Ify protein protocols
11) Paul's protocols
VII. Lipid Protocols
1) calculator
2) prep-sonicator
3) prep-extruder
4) Calcein release assay
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